Determination of Crustacean Allergen in Food Products by Sandwich ELISA
نویسندگان
چکیده
منابع مشابه
Development of DNA sensor crustacean allergen analysis in food products
Crustaceans is one of the most common causes of food allergies. Its persistency is life-threatening therefore, method to detect the presence of crustacean constituent in food products would be indispensable to ensure the safety. We have developed a simple and rapid method to detect the presence of crustacean residues in food products through the allergenic Pen gene. Detection method was based o...
متن کاملDevelopment of a Monoclonal Antibody-Based Sandwich ELISA for Peanut Allergen Ara h 1 in Food
We have established a highly sensitive sandwich enzyme-linked immunosorbent assay (ELISA) based on two monoclonal antibodies (mAb) to measure the content of the major peanut allergen Ara h 1 in foods. Two mAbs were selected out of 12 murine hybridoma cells secreting Ara h 1-specific antibody. Using mAb 6 as the capture antibody and HRP-labelled mAb 4 as the detection antibody, the limit of dete...
متن کاملQuantification of Pla or 3, a Platanus orientalis Allergen, Grown under Different Environmental Conditions, by Sandwich ELISA
Background: Platanus species are widely cultured around the world and considered an important cause of allergic reactions. In the present study, we developed a sandwich ELISA to quantify Pla or 3 allergen in P. orientalis pollen extracts grown near high-traffic roads and compared it to pollen extracts collected from rural areas as control. Methods: Pollen samples were collected from three po...
متن کاملQuantification assay for the major allergen of Cupressus sempervirens pollen, Cup s 1, by sandwich ELISA.
BACKGROUND The Cupressaceae are an important cause of pollinosis, particularly in Mediterranean countries. Cypress pollen allergenic extracts are difficult to produce since they have a low protein and a high carbohydrate content and consequently accurate standardization of these extracts is essential for diagnosis and immunotherapy. METHOD Natural Cup s 1 was purified by a combination of hydr...
متن کاملDetection of β-Lactamase Residues in Milk by Sandwich ELISA
β-Lactamase residues in milk represent a public health risk. The cylinder plate detection method, which is based on bacterial growth, is laborious and time consuming. In this study, 15 monoclonal antibodies (mAbs) were selected against Temoneira (TEM) 1 β-lactamase. A sandwich enzyme-linked immunosorbent assay (ELISA) based on an optimum mAb pair was developed and validated for the detection of...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nippon Shokuhin Kagaku Kogaku Kaishi
سال: 2007
ISSN: 1341-027X,1881-6681
DOI: 10.3136/nskkk.54.280